Manual of Techniques in Invertebrate Pathology, Second Edition

The second edition of Manual of Techniques in Invertebrate Pathology is written by an international group of experts that contribute a broad array of techniques for the identification, isolation, culture, bioassay, propagation, and storage of the major groups of entomopathogens. The manual provides general and specific background to experienced insect pathologists, biologists, and entomologists who work with pathogen groups that are new to them. It is also useful as a laboratory manual for courses in insect pathology and biological control and related areas of study. Safety testing of entomopathogens in mammals and complementary techniques for the preparation of entomopathogens are included as well as broader methods for the study of specimens such as microscopy and molecular techniques. This manual concentrates primarily on practical step-by-step aspects of the techniques, but also provides the reader with a short history, rationale for usage, guides to supplemental literature, plus recipes for media, fixatives, and stains.

• Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens
• New edition fully updated to address changes in the taxonomy of the vast majority of taxa
• Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques
• Provides extensive supplemental literature and recipes for media, fixatives and stains